Science Journal of Microbiology

March 2016, Volume 2016, ISSN: 2276-626X

Author(s) 2016. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

 

Capsular Polysaccharide from Klebsiella Pneumonia ATCC 70063: Isolation, Green Synthesis of Silver Nanoparticles and study of Antimicrobial Activity

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Gajendra Nath Maity1, Shaikh Rajesh Ali2, Subhabrata Mabhai3, Soumitra Mondal4*

1Department of Microbiology, Panskura Banamali College, Panskura, Purba Medinipur, Pin-721152, West Bengal, India.

2Department of Microbiology, Acharya Prafulla Chandra College, New Barrackpore, Kolkata-700131, West Bengal, India.

3 Department of Chemistry, Mahishadal Raj College, Mahishadal, Purba Medinipur, pin- 721628, West Bengal, India.

4Department of Chemistry, Panskura Banamali College, Panskura, Purba Medinipur, Pin-721152, West Bengal, India.

Accepted on February 08, 2016; Available Online 2 March, 2016

doi: 10.7237/sjmb/201

Abstract:

In modern research, the field of nanotechnology/nanoscience is the most interesting areas for chemists, biologists/microbiologists for their commercial demand and it emerges rapidly due to its wide application in chemistry, electronics ecology, and medicine as well as in biological fields. Economically preparations of silver nanoparticles using green synthesis path having biological entities are gradually increase. In the investigation, synthesis of Ag-nanoparticles were prepared by using capsular polysaccharide, isolated from Klebsiella pneumoniae ATCC 70063, and characterized by using UV-VIS spectrum band at 437 nm. The synthesized Ag-nanoparticles were generally found to be very effective as an antimicrobial agent against some important human pathogens such as E. coli ATCC 25922, K. pneumonia ATCC 70063, Salmonella typhi MTCC 734 and Salmonella typhimurium ATCC 14028, which are affecting mostly and causes serious problems to human beings. These Ag-nanoparticles particle have also sporocidal activity and also responsible for degradation of bacterial DNA, which may be another reason for bactericidal activity of the silver nanoparticles.

Keyword:Green synthesis, Capsular Polysaccharide (CPS), Silver nanoparticles (SNPs), endospore, antimicrobial activity, Human pathogenic bacteria.

 

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