Science Journal of Microbiology

March 2013, Volume 2013, ISSN: 2276-626X

Author(s) 2013. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

 

Exploitation of novel Actinomycetes isolated from Wetern ghats of Tamilnadu and production of Xylanase by Streptomyces spp.pds1

R.Ragunathan1, R. Padma Dhas2

1Department of Biotechnology, Maharaja Co Education Arts & Science College, Perundurai, Erode-52, Tamilnadu.

2Department of Biology, Dept. of Biology, University of Maldives, QAEC, Thinadhoo, Republic of Maldives.

Accepted 21 January 2013; Available Online 7 March, 2013

doi: 10.7237/sjmb/174

Abstract:

Western ghat is very rich in flora , fauna and microorganisms and it extent from Kerala, Karnataka and Tamilnadu. In the present totally 22 different soil actinomycets were isolated using Starch Casein Nitrate Agar plate method. After morphological identification one starain was identified by 16sRNA typing and identified as Streptomyces spp. pds1. And submitted to NCBI. The strain was used to produce Xylanase in production medium. The Xylanase activity was maximum on 96th hr of incubation (470U/mL). The optimum pH( 474 Um/L) and temperature were7.5 (468U/mL) and 40oC respectively. Further the Xylanase was purified by 70% acetone followed by dialysis and DEAE - Sephadex - 100 . The molecular weight was determined using SDS - PAGE and was found to be 60kDa.

Keyword:Western Ghats, Streptomyces, 16sRNA, Xylanase , SDS -PAGE)

 

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