Science Journal of Biochemistry
February 2016, Volume 2016, ISSN: 2276-6219
© Author(s) 2016. This work is distributed under the Creative Commons Attribution 3.0 License.
Evaluation of Urinary Liver-Type Fatty Acid Binding Protein As a Marker for Monitoring Type 2 Diabetic Nephropathy
1Faculty of Science; ALFaisaliah Campus; Biochemistry Department; King Abdulaziz University; Jeddah - Saudi Arabia.
2Medical Research Institute; Biochemistry Department; Alexandria University; Alexandria - Egypt.
3Internal Medicine Department, Medical Research institute, AlexandriaUniversity, Alexandria, Egypt
Accepted 01 February, 2016, 2013; Available Online Accepted 20 February, 2016
Background: Diabetic nephropathy (DN) is one of the most
important microvascular complications associated with type 2 diabetic
patients. It is known that tubulointerstitial damage plays an important
role in diabetic nephropathy .Therefore; it would potentially be beneficial
if albuminuria, as a marker of glomerular damage, could be
supplemented by a marker of tubular damage to provide a more
complete status of the kidney injury. Liver-type fatty acid-binding protein
(LFABP) is an intracellular carrier protein that is expressed in the
proximal tubules in the human kidney and participates in fatty acid
The present study aimed to evaluate the relation between the u-LFABP and some biochemical parameters to find their association with the occurrence of diabetic nephropathy. The study included 60 patients were divided into 3 groups diagnosed with type 2 diabetes (n=20/group) : group I: normoal-buminuria (ACR <30) ,group II: microalbuminuria ( ACR<30-300),group III:macroalbuminuria,( ACR >300) and control subjects comprised 20 non-diabetic healthy, age-matched subjects as the control group. All of the patients underwent a standardized laboratory evaluation including fasting and postprandial serum levels of glucose, glycated hemoglobin (HbA1c), kidney function, lipid profile, nonesterified fatty acids(NEFA)and serum and urinary levels of Liver-type Fatty Acid Binding Protein (L-FABP).
Our results revealed that UL-FABP levels were significantly higher in group I versus the control group and significantly increased according to the severity of diabetic nephropathy. Urinary L-FABP levels showed positive significant correlation with triglycerides and HDL-C, N-EFA and ACR and a negative significant correlation with age, duration of diabetes, HbA1c, serum creatinine, LDL-C., serum total cholesterol and systolic and diastolic blood. Urinary L-FABP provides a suitable biomarker for the early detection and monitoring of progression of diabetic nephropathy in clinical practice.
Keyword:Liver-type fatty acid-binding protein (LFABP), nonesterified fatty acids (NEFA), glycated hemoglobin (HbA1c), albumin creatinine ratio(ACR).