Science Journal of Biochemistry
April 2014, Volume 2014, ISSN: 2276-6105
© Author(s) 2014. This work is distributed under the Creative Commons Attribution 3.0 License.
Expression of Nuclear Factor Kappa P65 ;Ki-67 and Caspase-3 in Chemically -Induced Bladder Carcinogenesis
1Heba F. Gomaa; 2Saad M. El Gendy, 3Sabry Shaarawy and 4El-Aaser, A.A.
1Zoology Department, Faculty of science, Ain Shams University, Egypt.
2Prof. Dr. Saad M. EL-Gendy (Ph.D), Prof. Of Medical Biochem., Applied Medical Science College, Qassim University, KSA
3Prof Dr. Sabry Shaarawy (Ph.D) Prof. Of Medical Biochem., NCI, Cairo, Egypt,
Accepted 07 April, 2014, 2013; Available Online Accepted 27 April, 2014
The increased expression of NF-ΚB associated with carcinogenesis resulted in cell proliferation and stop apoptosis,in bladder carcinogenesis induced by dibutylamine / sodium nitrate mediated by e-coli infection. This study was designed to evaluate the potential protective effect of curcum in rats administered nitrosamine precursors; dibutylamine (DBA) and sodium nitrate (NaNO3); and infected with Escherichia coli (E. coli) and also to monitor changes in nuclear factor the Kappa B p65 (NF-ΚB p56) pathway and its downstream products, Bcl-2 and interleukin-6 (IL-6), in parallel with nitrosamine precursors, E. Coli and curcum treatment. Rats were divided into three groups (n = 25 each): Group I a normal control group, group II administered DBA/NaNO3 in drinking water and group III was administered DBA/NaNO3 in drinking water, infected with E. coli and receiving standard diet containing 1% curcum powder. and group IV received DBA/NaNO3 +E.coli+ curcum. Dibutylamine was given as 1000 ppm, NaNO3 was given as 2000 ppm in drinking water, infected by 0.1 ml saline containing suspension of E. coli in the bladder (approximately 2 X 106 organisms) and received standard diet containing 1% curcum powder mixed in the diet. Histopathological examination and immunohistochemical studies reflected that the curcum treated group featured a lower incidence of urinary bladder lesions, and lower levels of NF-ΚB, Bcl-2 and IL-6 and Ki67 protein than the group receiving nitrosamine precursor and infected with E. coli. These findings suggested that curcum may have a protective role during the process of bladder carcinogenesis by inhibiting the NF-ΚB pathway and its downstream products.
Keyword:Bladder carcinogenesis; Curcum; E.coli; DBA; NF-ΚB, Ki67; Immunohistochemistry; Caspase-3.