Science Journal of Biochemistry Volume 2012 (2012), January 2012
© Author(s) 2012. This work is distributed under the Creative Commons Attribution 3.0 License.
KINETIC CHARACTERISTICS OF UREASE ISOLATED FROM SELECTED BEANS SPECIES AS AFFECTED BY GERMINATION
1Ogunmolasuyi Adewoyin M,*1Evans C. Egwim
1Department of Biochemistry, Federal University of Technology, Minna, Nigeria
*1Department of Biochemistry, Federal University of Technology, Minna, Nigeria
Accepted 23 December 2011; Available Online 6 January, 2012
In this research four different beans (Glycine max, Mucuna pruriens, Kerstings'geocarpa and Vigna mungo) were germinated in order to evaluate the implication of germination on the kinetic parameters (Vmax and Km) of urease isolated from the various beans and compared to the non-germinated (control). The results showed that, Vmax and Km of urease from germinated beans increased significantly when compared (p<0.05) to urease from the control. This simply means that, urease from germinated beans demonstrated higher activity with lower substrate sensitivity when compared (p<0.05) to the control.
Keyword: Kinetics, Germination, Urease