Science Journal of Biochemistry Volume 2012 (2012), January 2012
ISSN:2276-6294

Author(s) 2012. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

 

KINETIC CHARACTERISTICS OF UREASE ISOLATED FROM SELECTED BEANS SPECIES AS AFFECTED BY GERMINATION

1Ogunmolasuyi Adewoyin M,*1Evans C. Egwim

1Department of Biochemistry, Federal University of Technology, Minna, Nigeria
*1Department of Biochemistry, Federal University of Technology, Minna, Nigeria

Accepted 23 December 2011; Available Online 6 January, 2012

Abstract:

In this research four different beans (Glycine max, Mucuna pruriens, Kerstings'geocarpa and Vigna mungo) were germinated in order to evaluate the implication of germination on the kinetic parameters (Vmax and Km) of urease isolated from the various beans and compared to the non-germinated (control). The results showed that, Vmax and Km of urease from germinated beans increased significantly when compared (p<0.05) to urease from the control. This simply means that, urease from germinated beans demonstrated higher activity with lower substrate sensitivity when compared (p<0.05) to the control.

Keyword: Kinetics, Germination, Urease

 

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